Active yeast ribosome preparation using monolithic anion exchange chromatography
نویسندگان
چکیده
منابع مشابه
Active yeast ribosome preparation using monolithic anion exchange chromatography
In vitro studies of translation provide critical mechanistic details, yet purification of large amounts of highly active eukaryotic ribosomes remains a challenge for biochemists and structural biologists. Here, we present an optimized method for preparation of highly active yeast ribosomes that could easily be adapted for purification of ribosomes from other species. The use of a nitrogen mill ...
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ژورنال
عنوان ژورنال: RNA Biology
سال: 2017
ISSN: 1547-6286,1555-8584
DOI: 10.1080/15476286.2016.1270004